Was born in Rossmoyne, Ohio. She was the daughter of John Henry Thompson and Clara Jane Gray, former slaves from Virginia.
Priscilla attended school in her home town, which was near Cincinnati, Ohio. She considered a career in teaching, however, ill health prevented her from pursuing this vocation. Instead, she devoted her energies to writing, publishing, and giving readings of her poetry.
Thompson's first book of poems, ETHIOPE LAYS, was published in 1900. In the introduction to this volume, Thompson says that she aims "as nearly as possible to picture the real side of my race, bringing in the foreground, their patience, fortitude and forbearance, devoid of that undertone of sarcasm, generally courted."
Her second volume, GLEANINGS OF QUIET HOURS, was published in 1907. She self-published and sold both volumes.
Thompson's poems deal with the themes of romantic love, death, religion, and race.